DWR Diagnostics Laboratory

Haematology and Haemostasis

Haematology

Test name Test details Sample requirement Test code Turnaround time

Haematology (canine and feline)

RBC, Hb, HCT, MCV, MCHC, MCH, WBC, differential count, platelet count, smear examination.

EDTA, blood film

CBC

Same day

Blood typing

Dog DEA1.1, Cat A, B or AB

EDTA

BT

Same day

Cross match

Major and minor cross match

EDTA from donor and recipient

CM

Same day

With an additional donor

CMA

Coombs’ test

Direct antiglobulin tests for IMHA

Canine

EDTA

COOM

up to 2 days

Feline COOC up to 2 days

Bone marrow aspirate

Cytological evaluation of aspirate with CBC

Slides + EDTA and blood film

BMAH

Same day

Added Perls’ Prussian blue stain for iron stores

PERL

2 days

With core biopsy

Cytology and histology of core biopsy with CBC

+ Core in formalin

BMAC

Core +3 days

Core only

Evaluation of bone marrow core biopsy

Core in formalin

BMC

3 days

Fibrosis stains

Massons and Reticulin stain on core for myelofibrosis

Core in formalin

MRSS

Haemoplasma PCR

Detects and quantifies 3 feline haemoplasma species- Mycoplasma haemofelis, Mycoplasma haemominutum & Mycoplasma turicensis

EDTA

HPCR

5–7 days

Iron + total iron binding capacity

In suspected iron deficiency anaemia

Serum

IRBC

3–5 days

Haemostasis

Test name

Test details

Sample requirement

Test code

Turnaround time

OSPT/APTT

Screen for coagulation factor function

Citrate plasma*

CLOT

Same day

Haemorrhage screen

CBC incl platelets and film exam, OSPT and APTT

EDTA, blood film and separated citrate plasma*

COAH

Same day

Full coagulation screen

CBC incl platelets and film exam, OSPT, APTT and D Dimers. To be run for dogs only as D Dimer is unreliable in cats

EDTA, blood film and separated citrate plasma*

COAG

Same day

D Dimers

For evaluation of DIC. To be run for dogs only- unreliable in cats

Citrate plasma*

DDIM

3 days

Angiostrongylus snap test

Screen for Angiostrongylus vasorum

Serum or plasma

ANGS

Same day

Von Willebrands factor

Measurement of vWF in suspected Von Willebrands disease

Citrate plasma*

VWF

7–10 days

Individual coagulation factors

Measurement of individual factors in suspected haemophilia/inherited coagulopathies

Citrate plasma*

Specify factor eg VIII

7–10 days

Antithrombin

Measured in cases of suspected deficiency due to renal loss in proteinuria

Citrate plasma*

ANTT

5 days

Vitamin K1 and Vitamin K epoxide

In evaluation of animals with suspected rodenticide poisoning (confirms vitamin K antagonism)

2ml Separated serum (spun gel tube) Protect from light

VITK

10 days

Warfarin & Coumarins

In suspected cases of intoxication

Heparinised blood or urine or >2ml stomach contents

WACO

5 days

* Handling of these samples is very important. Take the sample into citrate tube, fill exactly to the line, mix well, (20 inversions) then immediately separate the plasma and place in a plain tube with no gel or anticoagulant. Label as citrate plasma.